Coding

Part:BBa_K804005:Design

Designed by: Joe Ho   Group: iGEM12_British_Columbia   (2012-10-01)


DszC coding gene (from DszABC operon)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 882
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1032


Design Notes

2 PstI sites were removed using site-directed mutagenesis


Source

http://www.ncbi.nlm.nih.gov/nuccore/L37363.1 (GenBank: L37363.1)

References